Holbrook St Michaels suffered a 2-1 loss at home to Linby Colliery in the Central Midlands League South.
Damian Mann got both of the Linby goals, Andy Symcox replying for the home side.
In the Reserve Premier Division, Holbrook St Michaels Reserves won 4-1 at Thoresby Colliery Welfare.
Holbrook St Michaels are now 16th in the table with five points from their 14 league games so far.
Holbrook St Michaels are fifth in the Reserve Premier Division after five games, one position below Belper United Reserves.